In our test population, 46 out of the 52 SNPs (90%) were not polymorphic, having an allele frequency of 1 or 0 in the SNP assays. Overview of the polymorphic peptides contained in the MGF-file which was used for all database searches.
Das hat außerdem den Vorteil, dass Sie direkt auf die Werke im Volltext zugreifen können.Standardmäßig ist die Suchoberfläche von Springer auf Englisch gestellt. The HSPVdb, designed to reduce non-informative sequence information, outperforms the other databases.Next to the size of the database, relieving the accuracy condition from 1 to 50 ppm (Fig. In a mass spectrometry-based approach, the recorded tandem mass spectra are matched against hypothetical spectra generated from known protein sequence databases.
The value of our HSPV database is shown by identification of the majority of published polymorphic SNP- and/or ARF-derived epitopes from a mass spectrometry-based proteomics workflow, as well as by a large variety of polymorphic peptides identified as potential T cell epitopes in the HLA-ligandome presented by EBV cells.The HSPVdb consists of peptides derived from genomic sequence variations. Investigation of the frequency of SNPs in the dbSNP revealed that many SNPs are non-polymorphic “SNPs”. Proteomics 4:1985–1988Kessler JH, Melief CJ (2007) Identification of T-cell epitopes for cancer immunotherapy. Diese Werke sind nicht für Sie zugänglich. SNPs rs4848158, rs61378134, rs36023150, rs11540526, rs11554279, rs35958189, rs56013141, rs11541290, rs34422048, rs11541416, rs28659989, rs2070159, rs4261080, rs11557142, rs11555631, rs11479605, rs11541519, rs5030742, rs11548263 were analyzed using a KASPar assay with allele-specific primers labeled with VIC and FAM dyes, (KBioScience, Hoddesdon, UK). Two prophylactic vaccines have shown great promise in clinical trials. Dann finden Sie in Ihren Suchergebnissen nur Titel, auf die Sie vollen Zugriff haben.In der Seitenzeile von Springer können Sie Ihre Suchergebnisse weiter filtern. Das bedeutet, dass Juris nun dauerhaft für alle Hochschulmitglieder von zu Hause aus über den...Die Heimzugänge folgender Datenbänke stehen Ihnen nur noch bis zum 30.06.2020 zur Verfügung:Die Hochschulbibliothek der HSPV NRW hat zwei weitere Nationallizenzen freischalten lassenDer Wolters Kluwer Verlag hat seine online verfügbaren Inhalte erweitert This research was made possible by the financial assistance of the Landsteiner Foundation for Blood Transfusion Research. The complex MHC-peptide pool was prefractionated on a C18 RP-HPLC system (2 mm × 15 cm; Reprosil-C18-AQ 3 um; Dr. Maisch GmbH, Ammerbuch, Germany), using a gradient 0–60% A to B. First, a test set comprising approximately 30% of all MiHA known today, as listed in Table Our test set of known polymorphic peptides and allelic counterparts were synthesized and measured in standard on-line nanoHPLC/MS experiments, as in our normal proteomics workflow on HLA-ligands (Oliveira et al. Nat Methods 4:787–797, ReviewOliveira CC, van Veelen PA, Querido B, de Ru A, Sluijter M, Laban S, Drijfhout JW, van der Burg SH, Offringa R, van Hall T (2010) The nonpolymorphic MHC Qa-1Perkins DN, Pappin DJ, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. To reduce the size of this database, all duplicate amino acid sequences were deleted. Peripheral blood samples were obtained from healthy donors after approval by the Leiden University Medical Center Institutional Review Board and informed consent according to the Declaration of Helsinki.
Three heterozygosity categories were defined: 0/1, unknown, all others. Exp Hematol 31:1–10, ReviewThe UniProt Consortium (2008) The Universal Protein Resource (UniProt). L’impact de l’infection à papillomavirus (HPV) est considérable.
You can also search for this author in Overview of the polymorphic peptides contained in the MGF-file which was used for all database searches.